Isolation, production and partial purification of l-asparaginase from Pseudomonas aeruginosa by solid state fermentation

Abstract

L-asparaginase (L-asparagine amido hydrolase, E.C.3.5.1.1) is an extracellular enzyme having potential therapeutic applications. In the present study, production and partial purification of L-asparaginase fromPseudomonas aeruginosausing solid state fermentation was carried out. It is inferred thatthe bacterial growth was observed in cetrimide agar medium after 48 hours of incubation. The isolatePseudomonas aeruginosa was identified based on the morphological and biochemical characteristics. The isolated strainwas subjected to solid fermentation techniques along with Soya bean meal maker as substrate. The high amount of enzyme, Lasparaginase production was observed on optimal medium under 30 minutes incubation. Further, the enzyme activity was recorded maximum at 40oC. Similarly the molecular weight of enzyme was confirmed following the enzyme pattern analysis using SDS PAGE and found to be 39000 Daltons.

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